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KMID : 0370020010150000039
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Chung-Ang Journal of Pharmacal Sciences
2001 Volume.15 No. 0 p.39 ~ p.45
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Effect of Calcium Chelator on Silica-induced Inflammatory Response in RAW 264.7 Cells
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Abstract
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To investigate the action of calcium in silica-induced inflammatory response, we observed examined effect of calcium chelator on arachidonic acid (AA) release, reactive oxygen species (ROS) and peroxynitrite (PON) in RAW 264.7 cells stimulated by silica dose-dependently increased ［^3H］AA release, ROS and PON generation. Extracellular Ca^2+ chelator, EGTA, dose dependently inhibited silica-induced AA release and PON generation, but did not affect ROS generation. Intracellular Ca^2+ chelator, BAPTA, significantly inhibited ROS and PON generation but did not have any influence on AA release. These results suggest that PON generation is associated with both extracellular Ca^2+ influx and intracellular Ca^2+ release, whereas AA release and ROS generation is related to extracellular CA^2+ influx and intracellular Ca^2+ release in RAW 264.7 cells, respectively.
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